Commercial PYRO-sequencing on PyroMark Q24 and Q96 platforms

methylation

Pyrosequencing (unlike Sanger sequencing) reports the ratio of cytosine to thymine at each CpG site analyzed, which reflects the proportion of methylated DNA.Assay design is flexible, and can be performed in either orientation and on either strand. In addition, contiguous CpG sites are analyzed independently and within the same run, which enables assessment of sequence-wide methylation patterns while retaining details of position-specific methylation.

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SNP pyrosequencing

Insertions/Deletions and SNP ServicesBecause of the flexibility of primer placement in pyrosequencing reactions, virtually all genetic markers can be assayed. Alleles of variable loci are accurately quantified, and heterozygosity is easily resolved.

Because pyrosequencing delivers sequence information, various types of genetic variation can be evaluated: insertions and deletions, SNPs, single tandem repeats, and variable gene copy number. It is also possible to assay several contiguous sequence variants in a single run.

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Bacteria16S

Unlike hybridization techniques, pyrosequencing allows identification of a large number of species using a single conserved sequencing primer.Depending on assay design, pyrosequencing can be used to discriminate microbial species, types, and strains, or detect genetic mutations that confer resistance to antibiotics or antiviral drugs.

A growing number of assays specifically target commonly studied pathogenic species and strains of bacteria, viruses, and fungi.

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methylation

Detecting mutations is important in cancer management. Among several available detection methods, pyrosequencing is one of the most versatile. KRAS was one of the first genes found to be mutated in human cancer. Pyrosequencing provided sufficient analytical sensitivity and specificity to assess the mutation status in routine formalin-fixed CRC samples, even in tissues with low tumor cell content.Researchers were able to use commercially available pyrosequencing technologies (PyroMark Q24 KRAS and Q24 KRAS v2.0) to assess the mutation status for codon 61 in 96.8% of the cases.

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SNP pyrosequencing

Bacterial drug resistance markers often include heterogenic variation among several gene copies, which account for different drug resistance patterns.Pyrosequencing reliably reports the actual sequence of the locus. Thus, a single assay can detect a range of possible mutations including new and unexpected changes.